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Fluorescence-activated cell sorting-derived clones of Babesia bigemina show karyotype polymorphism
D M Estes1, C W Bailey, L Barnett
1Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Parasitology Research
|January 1, 1994
Abstract:
Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloning Babesia parasites. These qualities were examined by separating a mixed population of Babesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.