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Updated: Aug 17, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Humanized antibodies

G Winter1, W J Harris

  • 1Laboratory of Molecular Biology, MRC Centre for Protein Engineering, Cambridge, UK.

Trends in Pharmacological Sciences
|May 1, 1993
PubMed

Hybridoma technology enabled rodent monoclonal antibodies to be created against human pathogens and cells, but these had limited clinical utility. Protein engineering, reviewed her by Greg Winter and William Harris, is now generating antibodies for treatment of infectious disease, autoimmune disease and cancer by 'humanizing' rodent antibodies. Humanized antibodies have improved pharmacokinetics, reduced immunogenicity and have been used to clinical advantage.

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