Related Experiment Video

Updated: Aug 6, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

[The serine- and threonine dehydratases of brucellae]

V A Shirokov, S P Merinov

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |May 1, 1976
    PubMed

    More Related Videos

    Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)14:26

    Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

    Published on: April 6, 2019

    Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle08:37

    Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

    Published on: March 21, 2025

    Related Concept Videos

    The Proteasome02:18

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    Acid-Catalyzed Dehydration of Alcohols to Alkenes02:35

    Acid-Catalyzed Dehydration of Alcohols to Alkenes

    In a dehydration reaction, a hydroxyl group in an alcohol is eliminated along with the hydrogen from an adjacent carbon. Here, the products are an alkene and a molecule of water. Dehydration of alcohols is generally achieved by heating in the presence of an acid catalyst. While the dehydration of primary alcohols requires high temperatures and acid concentrations, secondary and tertiary alcohols can lose a water molecule under relatively mild conditions.
    Protein Folding Quality Check in the RER01:29

    Protein Folding Quality Check in the RER

    ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
    Export of Misfolded Proteins out of the ER01:32

    Export of Misfolded Proteins out of the ER

    After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
    Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation00:43

    Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation

    As shown in Figure 1, under acidic conditions, the β-hydroxy ketone undergoes dehydration via an E1 elimination reaction to form an enone.
    The Proteasome01:13

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...