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[Colored biomicrospheres for studies of cochlear microcirculation]
1Department of otorhinolaryngology, Union Hospital, Tong-Ji Medical University, Wuhan.
Zhonghua Er Bi Yan Hou Ke Za Zhi
|January 1, 1993
Summary
Toads red blood cells, stained red and blue, were used as biomicrospheres to measure cochlear blood flow (CBF) twice in guinea pigs. Results showed no significant difference in CBF between the two measurements, validating the method.
Area of Science:
- Physiology
- Biomedical Engineering
- Microcirculation Research
Context:
- Toads red blood cells have been utilized as biomicrospheres for studying cochlear microcirculation.
- Accurate measurement of cochlear blood flow (CBF) is crucial for understanding auditory system physiology and pathology.
Purpose:
- To evaluate the reliability of using differentially stained toad red blood cells as biomicrospheres for repeated measurements of cochlear blood flow (CBF) in the same animal.
- To determine if repeated injections of colored biomicrospheres affect the accuracy of CBF measurements.
Summary:
- Red blood cells from toads were stained with eosin (red) and azure blue, serving as distinct biomicrospheres.
- These colored biomicrospheres were injected intracardially into guinea pigs at two separate times, 4 minutes apart.
- Cochlear blood flow (CBF) was measured simultaneously using a laser Doppler system, with findings indicating no significant difference between the two injections.
- The study confirmed that the red and blue biomicrospheres were easily distinguishable from surrounding tissues, and an optimal injection count of 9 x 10^6/ml was established.
Impact:
- This study validates a reliable method for repeated, accurate cochlear blood flow (CBF) measurements using readily available biological material.
- The findings support the use of colored red blood cells as biomicrospheres in microcirculation studies, potentially reducing animal-to-animal variability.
- This technique offers a cost-effective and precise approach for investigating cochlear hemodynamics in various physiological and pathological conditions.