Zeta-protentials of intact cell monolayers determined by electro-osmosis

By measuring the electro-osmotic flow velocity of buffer through a glass capillary, on the inner surface of which cells have been grown, the zota-protential of these cells can be determined without the changes in zota-potential, viability and morphology caused by the chemical, enzymatic or mechanical pretreatment hitherto necessary for cell dispersion in the microelectrophoretic method. Cells grown in a monolayer inside the capillaries were: BGM, HEP-2, and RPMI 1846 (the last one also could be grown in suspension).