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Related Experiment Videos

Imipenem resistance in Enterobacter

K S Thomson1, C C Sanders, H Chmel

  • 1Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|August 1, 1993
PubMed
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Enterobacter strains resistant to imipenem produced a beta-lactamase enzyme that hydrolyzed the antibiotic. This enzyme, a group 1 cephalosporinase, offers a rapid detection method and contributes to imipenem resistance in Enterobacter.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Enzyme Kinetics

Background:

  • Enterobacter species are opportunistic pathogens frequently associated with healthcare-associated infections.
  • Antibiotic resistance, particularly to carbapenems like imipenem, is a growing global health concern.
  • Previous studies have indicated the role of beta-lactamases in Enterobacter antibiotic resistance.

Purpose of the Study:

  • To investigate the mechanism of reduced susceptibility to imipenem in clinical isolates of Enterobacter.
  • To characterize the beta-lactamase produced by these resistant isolates.
  • To evaluate a rapid detection method for imipenem-hydrolyzing enzymes.

Main Methods:

  • Isolation and characterization of Enterobacter strains from blood cultures.

Related Experiment Videos

  • Determination of antibiotic susceptibility profiles (imipenem, cephalosporins, penicillins, aztreonam).
  • Analysis of beta-lactamase production, including isoelectric focusing (pI) and enzyme activity assays.
  • Outer membrane protein analysis.
  • Three-dimensional and disk diffusion susceptibility testing.

    • Main Results:

    • Two Enterobacter isolates exhibited reduced susceptibility to multiple beta-lactam antibiotics, including imipenem.
    • Both isolates were unstable, yielding colony variants.
    • All variants produced a single, non-inducible Bush group 1 beta-lactamase (pI = 9.6) capable of hydrolyzing imipenem.
    • No significant differences in outer membrane proteins were observed between strains with varying imipenem resistance levels.
    • Three-dimensional tests combined with disk diffusion assays rapidly detected imipenemase production.

    Conclusions:

    • Overproduction of the Enterobacter group 1 cephalosporinase can contribute to imipenem resistance.
    • The characterized beta-lactamase is a key factor in the reduced susceptibility observed in these clinical isolates.
    • Rapid detection methods for imipenem-hydrolyzing enzymes are valuable for clinical settings.