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32P-postlabelling analysis of alkylphosphotriesters in DNA
C P Saris1, S J Damman, A M van den Ende
1Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
IARC Scientific Publications
|January 1, 1993
Abstract:
We have developed a highly sensitive 32P-postlabelling assay for the detection of alkylphosphotriesters in DNA using selective enzymatic DNA hydrolysis, sample preparation by C18 Sep-Pak solid phase extraction and product analysis by HPLC. The assay provides information on the quantity of the alkylphosphotriesters and gives some clues with respect to their identity.