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Related Experiment Videos

Viscosity sensing with lamb-wave microsensor: dimethylsulfoxide solution viscosity as a function of temperature

T K Eto1, B J Costello, S W Wenzel

  • 1Department of Mechanical Engineering, University of California at Berkeley 94720.

Journal of Biomechanical Engineering
|August 1, 1993
PubMed
Summary

A new ultrasonic microsensor accurately measures the viscosity of dimethylsulfoxide (Me2SO) solutions. This viscosity data as a function of temperature is crucial for understanding ice nucleation during cryopreservation.

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Area of Science:

  • Physical Chemistry
  • Materials Science
  • Biophysics

Background:

  • Cryopreservation relies on cryoprotectants like dimethylsulfoxide (Me2SO) to prevent ice crystal formation in biological materials.
  • Understanding the physical properties of cryoprotectant solutions at low temperatures is critical for optimizing preservation protocols.
  • The temperature-dependent viscosity of solutions influences ice nucleation probability, a key factor in cryopreservation success.

Purpose of the Study:

  • To experimentally determine the viscosity of dimethylsulfoxide (Me2SO) aqueous solutions across a range of concentrations and temperatures.
  • To validate the use of a novel low-velocity ultrasonic Lamb-wave microsensor for precise viscosity measurements in small volumes.
  • To provide essential viscosity-temperature data for Me2SO solutions relevant to cryopreservation applications.

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Main Methods:

  • Utilized a microsensor based on low-velocity ultrasonic Lamb waves for viscosity measurements.
  • Integrated the microsensor with a temperature-controlled stage to enable temperature-dependent analysis.
  • Prepared and tested 1M, 3M, and 5M aqueous solutions of dimethylsulfoxide (Me2SO).

Main Results:

  • Successfully measured the viscosity of 1M, 3M, and 5M Me2SO aqueous solutions as a function of temperature.
  • Demonstrated the capability of the ultrasonic Lamb-wave microsensor for accurate viscosity determination in small sample volumes.
  • Obtained detailed temperature-dependence profiles for the viscosity of the tested Me2SO solutions.

Conclusions:

  • The ultrasonic Lamb-wave microsensor is a viable tool for characterizing the rheological properties of cryoprotectant solutions.
  • The acquired viscosity-temperature data for Me2SO solutions can inform models predicting ice nucleation probability.
  • This research contributes to the fundamental understanding required for improving cryopreservation techniques for biological materials.