Determination of Roxarsone in feeds using solid phase extraction and liquid chromatography with ultraviolet detection

Area of Science:

• Analytical Chemistry
• Food Science
• Veterinary Pharmacology

Background:

• Roxarsone is an organoarsenic feed additive used in poultry production.
• Accurate quantification of Roxarsone in feed is crucial for regulatory compliance and animal health.
• Existing analytical methods may require improvement in terms of efficiency and accuracy.

Purpose of the Study:

• To develop and validate a robust method for Roxarsone detection and quantitation in poultry feed.
• To establish a reliable analytical procedure for routine quality control of animal feed.

Main Methods:

• Sample preparation involving phosphate buffer extraction.
• Determination using solid-phase extraction (SPE) cleanup.
• Quantification via reversed-phase high-performance liquid chromatography (RP-HPLC).

Main Results:

• The developed liquid chromatography method demonstrated effective detection and quantification of Roxarsone.
• High recoveries were achieved for both sample spikes and fortified field samples.
• Results closely correlated with those obtained using a standard spectrophotometric method, indicating high accuracy.

Conclusions:

• The presented liquid chromatography method provides a reliable and accurate means for analyzing Roxarsone in poultry feed.
• This method is suitable for routine analysis and contributes to ensuring the safety and quality of animal feed.
• The validated method offers an alternative to traditional spectrophotometric techniques.