Antibodies to fluorylsulfonylbenzoyladenosine permit identification of protein kinases

Antibodies were raised to fluorylsulfonylbenzoyladenosine (FSBA) coupled to carrier proteins. Affinity purified sera are shown to specifically detect protein kinase C-beta 1 treated with FSBA. Immunodetection is specific to modification at the ATP binding site as judged by the ability of ATP to block subsequent detection. The antisera are shown to react with a number of U937 cell proteins fractionated on Mono Q and treated with FSBA; the majority of these immunoreactions are prevented by inclusion of ATP in the FSBA treatments. One of these is positively identified as PKC-beta 1.