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Related Experiment Videos

Histaminase release from human granulocytes

R S Zeiger, F J Twarog, H R Colten

    The Journal of Experimental Medicine
    |October 1, 1976
    PubMed
    Summary
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    Human granulocytes release histaminase (an enzyme that breaks down histamine) in response to inflammation triggers. This release mechanism, dependent on cell metabolism and divalent cations, suggests a role in modulating histamine effects at inflammatory sites.

    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • Histaminase (EC-1.4.3.6) is a key enzyme in histamine catabolism.
    • Human granulocytes contain histaminase and play a role in inflammatory responses.

    Purpose of the Study:

    • To investigate the release of histaminase from human granulocytes.
    • To understand the mechanisms and conditions regulating histaminase release.

    Main Methods:

    • Human granulocytes were stimulated in vitro using opsonized zymosan and the calcium ionophore A-23187.
    • Histaminase and beta-glucuronidase release were measured under various conditions (temperature, divalent cations).
    • Inhibitory effects of metabolic and microtubule-disrupting agents were assessed.

    Main Results:

    Related Experiment Videos

    • Opsonized zymosan and ionophore A-23187 induced dose-dependent histaminase release from granulocytes.
    • Release was temperature-dependent, required divalent cations (Ca2+, Mg2+), and completed within 30 minutes.
    • Total cellular histaminase was released, unlike lysosomal beta-glucuronidase (25% release with zymosan).
    • Release was inhibited by agents affecting cell metabolism and microtubules.

    Conclusions:

    • Human granulocytes can release histaminase, potentially modulating histamine effects at inflammation sites.
    • Granulocyte histaminase release provides a model for studying granulocyte function and secretion mechanisms.