Related Experiment Video
Updated: Aug 10, 2026

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
Determination of dopamine and its metabolites in microdialysates by capillary liquid chromatography with
1Institute of Macromolecular Compounds, Russian Academy of Science, Petersburg.
Abstract:
A liquid chromatographic method with packed fused-silica capillary columns and amperometric detection for the determination of trace amounts of catecholamines in microdialysis samples has been developed. The analysis time for dopamine and the products of its metabolism does not exceed 10 min. The minimum detectable dopamine concentration in a 2-microliters sample is 0.05 microgram/l. The relative standard deviation in concentration determinations is +/- 0.03 (n = 10). The small sample volume required and the speed of analysis make it possible to analyse biologically important compounds with a rapidly changing concentration.
More Related Videos
Related Concept Videos
Dialysis
High-Performance Liquid Chromatography: Types of Detectors
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

