Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleoside triples from the group I intron

M Chastain1, I Tinoco

  • 1Department of Chemistry, University of California, Berkeley 94720.

Biochemistry
|December 28, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biophysical analysis of nucleic acids.

Current protocols in nucleic acid chemistry·2008
Same author

Force unfolding kinetics of RNA using optical tweezers. II. Modeling experiments.

Biophysical journal·2007
Same author

Verification of the Crooks fluctuation theorem and recovery of RNA folding free energies.

Nature·2005
Same author

The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.

Biochemical Society transactions·2004
Same author

[A 65-year-old woman with mediastinal enlargement].

Revista clinica espanola·2004
Same author

Efficacy of directly observed treatment of HIV infection: experience in AIDS welfare homes.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2004
Same journal

Bilirubin Reductase from <i>Mediterraneibacter gnavus</i>: Positional Reduction Preferences and Transient-State Analysis.

Biochemistry·2026
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
See all related articles

Researchers discovered a novel nucleoside triple interaction in group I introns. This interaction may align catalytic domains rather than directly participating in catalysis, impacting intron function.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Group I introns are catalytic RNAs involved in self-splicing.
  • The P4/P6 region is crucial for the catalytic activity of these introns.
  • A proposed base-triple domain within P4/P6 has been hypothesized to play a structural role.

Purpose of the Study:

  • To characterize the structure of oligonucleotides modeling the P4/P6 base-triple domain.
  • To investigate the formation and structural features of nucleoside triples in this region.
  • To determine the potential role of nucleoside triples in group I intron catalysis.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy was used to analyze oligonucleotide structures.
  • Oligonucleotides with conserved and non-conserved sequences were synthesized and studied.

Related Experiment Videos

  • Structural data were interpreted to identify specific interactions and conformations.

    • Main Results:

    • NMR data revealed a novel 'nucleoside triple' interaction involving single-stranded nucleotides and an adjacent helix's minor groove.
    • Conserved sequences formed these nucleoside triples, while non-conserved sequences did not.
    • Surprisingly, nucleoside triples formed from conserved sequences exhibited distinct structures.

    Conclusions:

    • The formation of nucleoside triples is sequence-dependent and specific to conserved regions within group I introns.
    • The structural diversity of these triples suggests they may not be directly catalytic.
    • Nucleoside triples likely function by organizing helical domains within the intron's catalytic core, influencing overall intron structure and function.