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Protein kinase A-mediated phosphorylation of the T-cell surface antigen CD27

K Sugita1, J D Dasgupta, Y Nojima

  • 1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.

Immunology
|October 1, 1993
PubMed

Insights

CD27, a T-cell surface antigen, is rapidly phosphorylated during T-cell activation. This process is primarily mediated by cyclic AMP-protein kinase A, highlighting a novel signaling pathway for T-cell surface molecules.

Area of Science:

  • Immunology
  • Cell Signaling

Background:

  • CD27 is a T-cell surface antigen belonging to a receptor family including TNF receptors and Fas.
  • The precise function of CD27 in T-cell activation remains largely undefined.
  • Experimental evidence suggests CD27 plays a significant role in T-cell activation processes.

Purpose of the Study:

  • To investigate the phosphorylation of CD27 during T-cell activation.
  • To identify the key kinases involved in CD27 phosphorylation.
  • To elucidate the signaling pathways regulating CD27 phosphorylation.

Main Methods:

  • Stimulation of T-cells with cyclic AMP-inducing agents (forskolin, 8-bromo-cAMP) and PMA.
  • Analysis of CD27 phosphorylation levels.
  • Inhibition of kinase activity using H-8 (cyclic nucleotide-dependent kinase inhibitor) and staurosporine (protein kinase C inhibitor).
  • T-cell activation via CD2 or CD3 pathways.

Main Results:

  • CD27 undergoes rapid hyperphosphorylation upon stimulation with cyclic AMP-inducing agents and PMA.
  • T-cell activation via CD2/CD3 pathways leads to increased CD27 phosphorylation.
  • CD27 phosphorylation is significantly suppressed by H-8, but only slightly by staurosporine.
  • These findings implicate protein kinase A as a key regulator of CD27 phosphorylation.

Conclusions:

  • CD27 phosphorylation is regulated by the cyclic AMP-protein kinase A pathway.
  • This study identifies CD27 as the first T-cell surface antigen phosphorylated via this specific pathway.
  • The findings provide new insights into the signaling mechanisms governing T-cell activation and CD27 function.

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