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An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures
I Vassias1, S Perol, L Coulombel
1Laboratory of Microbiology, Saint-Louis Hospital, Paris, France.
Journal of Virological Methods
|October 1, 1993
Abstract:
An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.