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Labeling human lymphocytes with mercury-197

G B Saha, E T Schell, P A Farrer

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |January 1, 1977
    PubMed
    Summary
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    Researchers developed a simple method to label human lymphocytes with mercury-197 (Hg-197). This technique enhances labeling yield, particularly when cells are stimulated, with the tracer concentrating in the nucleus and cell membrane.

    Area of Science:

    • Biomedical research
    • Cell biology
    • Radiochemistry

    Background:

    • Human lymphocytes are crucial for immune responses.
    • Efficient cell labeling is vital for diagnostic and research applications.

    Purpose of the Study:

    • To establish a straightforward method for labeling human lymphocytes using mercury-197 (Hg-197).
    • To optimize labeling conditions and assess tracer distribution within cells.

    Main Methods:

    • Isolation and purification of human lymphocytes using the Ficoll-Hypaque method.
    • Incubation of viable lymphocytes with Hg-197 under varying time and temperature conditions.
    • Subcellular fractionation to determine tracer localization.

    Main Results:

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    • Labeling yield increased with cell number, reaching approximately 70% for 10 million cells.
    • Optimal labeling achieved after 2 hours at 37°C.
    • Phytohemagglutinin stimulation increased labeling yield by 1.5 times.
    • Hg-197 predominantly localized in the nucleus (60%) and cell membrane.

    Conclusions:

    • A simple and effective method for Hg-197 labeling of human lymphocytes was developed.
    • Labeling efficiency is influenced by cell concentration and stimulation.
    • The tracer's distribution suggests potential for studying nuclear and membrane-associated cellular processes.