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Monoclonal antibodies suitable for plum pox virus determination
I Hilgert1, D Cikánek, H Kristofová
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Hybridoma
|April 1, 1993
Summary
Monoclonal antibodies (MAbs) were developed for plum pox virus (PPV) detection. These MAbs offer comparable sensitivity and effectiveness to commercial kits for diagnosing PPV in stone fruits.
Area of Science:
- Plant Virology
- Immunology
- Biotechnology
Background:
- Plum Pox Virus (PPV) is a significant pathogen affecting stone fruit crops worldwide.
- Accurate and sensitive diagnostic tools are crucial for managing PPV outbreaks.
- Existing diagnostic methods have limitations in sensitivity or specificity.
Purpose of the Study:
- To develop and characterize monoclonal antibodies (MAbs) for the specific detection of Plum Pox Virus (PPV).
- To evaluate the diagnostic performance of these MAbs in comparison to existing methods.
Main Methods:
- Immunization of BALB/c mice with purified PPV-W isolate.
- Hybridoma technology for MAb production (fusion with Sp2/0-Ag14 myeloma cells).
- Indirect ELISA for screening antibody production and differentiating PPV isolates (W, A, D, M).
Main Results:
- Six stable hybridoma lines producing IgG class MAbs against PPV were successfully established.
- The developed MAbs demonstrated the ability to distinguish between different PPV isolates.
- Comparative studies showed MAbs had comparable sensitivity (10-50 ng/ml) and diagnostic performance to commercial kits.
Conclusions:
- Monoclonal antibodies provide a sensitive and specific tool for PPV diagnostics.
- These MAbs are effective for routine diagnosis of PPV in plums, peaches, and apricots.
- The developed MAbs offer a viable alternative to current commercial diagnostic kits.