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Determination of khellin in serum by gas chromatography
A S Carlin1, J E Simmons, S K el-Arini
1Biopharmaceutics Research Branch, Food and Drug Administration, Washington, DC 20204.
Journal of Chromatography
|May 5, 1993
Abstract:
A rapid gas chromatographic assay has been developed for the separation and determination of khellin in human serum. Using a DB-17 capillary column and a simple chloroform extraction, khellin and an internal standard, trioxsalen, were separated from endogenous substances without further clean-up. Spiked serum samples in the range 0.11-1.1 micrograms/ml were assayed and a linear calibration curve obtained.