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Reduction of nitric oxide by denitrifying bacteria
R Shanmugasundram1, C M Lee, K L Sublette
1Center for Environmental Research and Technology, University of Tulsa, OK 74104.
Applied Biochemistry and Biotechnology
|January 1, 1993
Summary
Two bacteria, Paracoccus denitrificans and Pseudomonas denitrificans, effectively remove nitric oxide (NO) using various carbon sources. This suggests a potential for cost-effective microbial treatment of nitrogen oxides (NOx).
Area of Science:
- Microbiology
- Environmental Science
- Biotechnology
Background:
- Denitrifying bacteria play a crucial role in nitrogen cycling.
- Nitric oxide (NO) is a key intermediate in denitrification.
- Effective biological treatment methods for nitrogen oxides (NOx) are needed.
Purpose of the Study:
- To investigate the capability of heterotrophic denitrifying bacteria to utilize nitric oxide (NO).
- To identify suitable carbon and energy sources for NO reduction by these bacteria.
- To assess the potential for microbial NOx disposal.
Main Methods:
- Culturing of Paracoccus denitrificans and Pseudomonas denitrificans.
- Exposure of bacterial cultures to a gas feed containing 0.50% nitric oxide (NO).
- Utilization of succinate, yeast extract, and pretreated municipal sewage sludge as substrates.
Main Results:
- Complete removal of nitric oxide (NO) from the gas feed was achieved by both bacterial species.
- Successful utilization of succinate, yeast extract, and treated sewage sludge as carbon and energy sources.
- Demonstrated efficient NO reduction under tested conditions.
Conclusions:
- Nitric oxide (NO) reduction is likely a common characteristic among denitrifying bacteria.
- Microbial treatment processes for nitrogen oxides (NOx) show economic viability.
- Paracoccus denitrificans and Pseudomonas denitrificans are effective agents for NO removal.