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Selective media for detecting and enumerating foodborne yeasts
1Department of Food Science and Technology, University of Georgia, Griffin 30223-1797.
International Journal of Food Microbiology
|June 25, 1993
Summary
Selecting the right yeast culture media is crucial for accurate detection and enumeration in foods. Various specialized media, like dichloran rose Bengal chloramphenicol agar, offer superior performance for spoilage yeasts and xerophiles.
Area of Science:
- Food Microbiology
- Microbial Detection and Enumeration
Background:
- No single medium effectively detects, isolates, or enumerates all yeasts across diverse food matrices.
- Traditional acidified media are often less effective than antibiotic-supplemented options for most spoilage yeasts.
Purpose of the Study:
- To review and highlight effective microbiological media for yeast detection and enumeration in foods.
- To identify specialized media for specific yeast types, including spoilage, xerotolerant, and wild yeasts.
Main Methods:
- Evaluation of various selective and differential microbiological media.
- Comparison of antibiotic-supplemented media against acidified media.
- Assessment of media for xerotolerant, preservative-resistant, and wild yeasts.
Main Results:
- Antibiotic-supplemented media (e.g., dichloran rose Bengal chloramphenicol agar) excel in enumerating most spoilage yeasts.
- Dichloran glycerol (18%) agar and malt extract yeast extract glucose (up to 60%) are effective for xerotolerant yeasts and xerophiles.
- Specialized media like lysine agar and ethanol sulfite yeast extract agar aid in differentiating wild yeasts and specific genera like Saccharomyces.
Conclusions:
- A range of specialized media are superior to general-purpose media for specific yeast detection and enumeration in foods.
- There is an ongoing need for novel media to isolate diverse yeast groups under specific food conditions.