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Updated: Aug 8, 2026
04:36
Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Immuno-assay techniques for detecting yeasts in foods
1Laboratory of Microbiology, Wageningen Agricultural University, The Netherlands.
International Journal of Food Microbiology
|June 25, 1993
Summary
This study reviews immunoassays for yeast cell wall antigens, focusing on extracellular, thermostable yeast antigens (EPS). Sandwich ELISA effectively detected EPS from Saccharomyces cerevisiae and Stephanoascus ciferrii.
Area of Science:
- Microbiology
- Immunology
- Biochemistry
Background:
- Yeast cell wall antigens, particularly extracellular, thermostable yeast antigens (EPS), are released into growth media.
- Immunoassays are crucial for detecting and characterizing these yeast-derived molecules.
Purpose of the Study:
- To review and evaluate immunoassays for detecting yeast cell wall antigens.
- To assess the specificity and sensitivity of various immunoassay formats for different yeast species.
Main Methods:
- Literature review of immunoassays for yeast antigens.
- Development and testing of sandwich ELISA for specific yeast EPS.
- Evaluation of competitive ELISA and latex agglutination tests.
Main Results:
- Sandwich ELISA specifically and sensitively detected EPS from Saccharomyces cerevisiae and Stephanoascus ciferrii.
- EPS ELISA lacked specificity for tested basidiomycetous yeasts.
- Geotrichum candidum EPS ELISA was genus-specific but not sensitive.
- Zygosaccharomyces bailii EPS was detected by competitive ELISA but not sandwich ELISA or latex agglutination.
Conclusions:
- Sandwich ELISA is effective for detecting specific yeast EPS from certain species.
- Immunoassay performance varies significantly depending on the yeast species and antigen.
- Competitive ELISA offers an alternative for detecting specific yeast antigens like Zygosaccharomyces bailii EPS.
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