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Non-injection methods for the production of embryonic stem cell-embryo chimaeras
S A Wood1, N D Allen, J Rossant
1Department of Molecular Embryology, Max-Planck Institut für Immunbiologie, Freiburg, Germany.
Nature
|September 2, 1993
Abstract:
The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.