Cytotoxicity testing of two CAPD dialysis fluids in a model system of quiescent fibroblasts

Indications of some bio-incompatibility of fluids used for continuous ambulatory peritoneal dialysis (CAPD) have been found previously. We have tested cytotoxicity of an frequently used dialysis fluid, Dianeal, and a new bicarbonate-based dialysis fluid with a pH of 7.4, called 87b, in a model system of quiescent fibroblasts. The ATP level of the cells and release of lactate dehydrogenase was followed during incubation in the dialysis fluids. A marked cytotoxicity of Dianeal was found mainly due to a low pH (5.0-5.5) and to a minor degree because of the lack of potassium and glutamine. 87b was less cytotoxic but it also lacked potassium and glutamine.