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Mass spectrometric evidence for a disulfide bond in aequorin regeneration
1PRESTO, JRDC, Solar Energy Research Group, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
FEBS Letters
|October 18, 1993
Abstract:
Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys145 and Cys152 and that the reduction of this bond is involved in the regeneration of aequorin.