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Neurotropism of mouse-adapted haemagglutinating encephalomyelitis virus
K Yagami1, Y Izumi, N Kajiwara
1Laboratory Animal Research Center, University of Tsukuba, Ibaraki, Japan.
Abstract:
The propagation of a mouse-adapted strain (67N) of haemagglutinating encephalomyelitis virus in infected mice and murine cells was examined by viral re-isolation and immunostaining. Viral propagation was strictly limited to the neurons and to an established line of neuroblastoma cells in in-vivo and in-vitro experiments. These results provide adequate evidence that this virus is neurotropic.
Insights
Haemagglutinating encephalomyelitis virus (HEV) propagation was studied in mice and neuronal cells. The virus exclusively infected neurons, confirming its neurotropic nature.
Area of Science:
- Virology
- Neuroscience
- Infectious Diseases
Background:
- Haemagglutinating encephalomyelitis virus (HEV) is an important pathogen in swine.
- Understanding HEV tropism is crucial for disease control and prevention.
Purpose of the Study:
- To investigate the neurotropic potential of a mouse-adapted HEV strain (67N).
- To determine the cellular targets of HEV infection in vivo and in vitro.
Main Methods:
- Viral re-isolation from infected mice and murine cell cultures.
- Immunostaining techniques to detect viral antigen presence.
Main Results:
- HEV (67N) successfully propagated in experimentally infected mice.
- Viral propagation was exclusively observed in neuronal cells.
- HEV replicated in an established murine neuroblastoma cell line.
Conclusions:
- The study provides strong evidence for the neurotropic nature of the investigated HEV strain.
- HEV specifically targets neuronal cells, highlighting its potential to cause neurological disease.