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Updated: Aug 15, 2026

Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
Published on: July 5, 2017
[NADPH-D reactive choroid ganglion cells in the human]
A Bergua1, A Jünemann, G O Naumann
1Augenklinik mit Poliklinik, Universität Erlangen-Nürnberg.
Background:
The demonstration of uveal ganglion cells has been investigated so far by silver impregnation, rather complicated neurohistochemical methods and by electron microscopy. We present the first results of a simple method in flat-preparation of the human choroid.
Material And Methods:
Donors' eyes for cornea transplantation, 5 men, 3 women, aged 45 +/- 13 years, with postmortal time of preparation of 12 +/- 9 h (1 h-24 h) were used. After removal of the cornea with scleral ring, flat-preparations of the choroid were treated by the standard Nicotinamide-Adenine-Dinucleotide-Phosphate (NADPH)-diaphorase reaction, mounted on coated slides in Kayser's glycerol-gelatin and observed by light microscope.
Results:
NADPH-diaphorase reaction reveals abundant aggregates of ganglion cells, with nets of dendrites and axons in the otherwise unstained flat-preparation of the human choroidea. They are more abundant in the layer close to the sclera.
Conclusion:
NADPH-diaphorase reaction is a simple method for the detection of ganglion cells in the human choroid. Their unexpectedly high number suggests yet unknown ganglional functions in the uvea.
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