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The GenePrint Light method for Southern transfer and chemiluminescent detection of human genomic DNA
C J Sprecher1, L R Liss, J W Schumm
1R&D Department, Promega Corporation, Madison, WI 53711.
Biotechniques
|February 1, 1993
Abstract:
A simple, fast and sensitive method to perform Southern transfer and hybridization with nonradioactive detection of genetic loci is described. With a 10-ng sample of human genomic DNA, alleles of the D2S44 locus can be detected within 7 h of completing gel electrophoresis.