Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sodium is elevated in mdx muscles: ionic interactions in dystrophic cells

J F Dunn1, N Bannister, G J Kemp

  • 1MRC Biochemical and Clinical Magnetic Resonance Unit, Dept. of Biochemistry, University of Oxford, UK.

Journal of the Neurological Sciences
|January 1, 1993
PubMed
Summary

Intracellular sodium concentration (Nai) is elevated in mdx mice, a model for Duchenne muscular dystrophy. This finding suggests potential cellular regulation defects contributing to muscle disease progression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced Cerebrovascular Oxygenation in Individuals with Post-Acute COVID-19 Syndrome (PACS) ("long COVID").

Advances in experimental medicine and biology·2023
Same author

Non-invasive Detection of Persistent Cortical Hypoxia in Multiple Sclerosis Using Frequency Domain Near-Infrared Spectroscopy (fdNIRS).

Advances in experimental medicine and biology·2022
Same author

Differential regulation of STP, LTP and LTD by structurally diverse NMDA receptor subunit-specific positive allosteric modulators.

Neuropharmacology·2021
Same author

Near-Infrared Spectroscopy Reveals Brain Hypoxia and Cerebrovascular Dysregulation in Primary Biliary Cholangitis.

Hepatology (Baltimore, Md.)·2019
Same author

Initiating and imaging the coherent surface dynamics of charge carriers in real space.

Nature communications·2016
Same author

Pregnancies complicated by preeclampsia and non-preeclampsia-related nephrotic range proteinuria.

Obstetric medicine·2016

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Physiology

Background:

  • Cellular sodium regulation is crucial for muscle function.
  • Evidence suggests abnormalities in sodium handling in muscular dystrophy.

Purpose of the Study:

  • To investigate intracellular sodium concentration (Nai) in mdx mice, an animal model of Duchenne muscular dystrophy.
  • To explore potential mechanisms and consequences of altered sodium levels in muscular dystrophy.

Main Methods:

  • Measurement of intracellular sodium concentration (Nai) using microelectrode technique in isolated diaphragm.
  • Calculation of Nai in gastrocnemius muscle using extra- and intracellular volumes and sodium concentrations.

Main Results:

  • Significantly elevated Nai in diaphragm muscles of mdx mice compared to controls (23.5 mM vs 13.0 mM).

Related Experiment Videos

  • Similarly, elevated Nai in gastrocnemius muscles of mdx mice compared to controls (24 mM vs 13 mM).

    • Conclusions:

    • The study demonstrates abnormal intracellular sodium accumulation in mdx mouse muscles.
    • This elevation may stem from reduced Na/K ATPase activity or increased sodium permeability.
    • Elevated Nai could impair cell volume control, contributing to Duchenne muscular dystrophy pathology.