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Human plasma phospholipid transfer protein causes high density lipoprotein conversion
M Jauhiainen1, J Metso, R Pahlman
1National Public Health Institute, Department of Biochemistry, Helsinki, Finland.
The Journal of Biological Chemistry
|February 25, 1993
Summary
Human plasma phospholipid transfer protein (PLTP) alters high density lipoprotein (HDL) particle sizes in vitro. PLTP converts HDL3 into larger and smaller HDL particles, suggesting a role in HDL remodeling.
Area of Science:
- Lipid metabolism
- Cardiovascular research
- Protein biochemistry
Background:
- High density lipoprotein (HDL) particle size is a key determinant of its atheroprotective function.
- The role of phospholipid transfer protein (PLTP) in modulating HDL subspecies remains incompletely understood.
Purpose of the Study:
- To investigate the effect of purified human plasma phospholipid transfer protein (PLTP) on the particle size distribution of human high density lipoprotein (HDL).
Main Methods:
- Incubation of human HDL3 with purified PLTP in vitro.
- Analysis of HDL particle size distribution using gradient gel electrophoresis and high-resolution gel filtration.
- Characterization of PLTP purity and assessment of anti-CETP antibody activity.
Main Results:
- PLTP induced the conversion of homogeneous HDL3 into two distinct populations: larger (10.9 nm) and smaller (7.8 nm) HDL particles.
- The extent of HDL conversion was dose- and time-dependent on PLTP.
- An anti-CETP antibody did not affect PLTP-mediated HDL conversion, confirming PLTP's distinct activity.
Conclusions:
- Human plasma PLTP possesses the ability to convert HDL3 into heterogeneous populations of larger and smaller HDL particles.
- PLTP may act as a conversion factor influencing HDL remodeling in vivo.
- Further studies are needed to elucidate the mechanisms and physiological significance of PLTP-mediated HDL conversion.