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Intermediate filament structure and assembly

M Stewart1

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Current Opinion in Cell Biology
|February 1, 1993
PubMed
Summary
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Intermediate filaments assemble from coiled-coil molecules on a helical lattice. Molecular packing imperfections influence filament assembly and dynamics, complicating mutagenesis data interpretation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Structural Biology

Background:

  • Intermediate filaments are cytoskeletal polymers crucial for cellular structure and mechanical resilience.
  • Their assembly involves complex hierarchical structures, from individual molecules to higher-order filaments.
  • Understanding filament assembly is key to deciphering cellular mechanics and disease pathologies.

Purpose of the Study:

  • To elucidate the structural principles governing intermediate filament assembly.
  • To investigate the role of molecular packing in filament formation and dynamics.
  • To clarify the interpretation of mutagenesis data in the context of filament structure.

Main Methods:

  • Analysis of intermediate filament molecular structure.
  • Investigation of oligomer formation and filament nucleation/elongation processes.

Related Experiment Videos

  • Examination of mutagenesis data related to rod domain termini.

    • Main Results:

    • Intermediate filaments are built from two-chain alpha-helical coiled-coil molecules on an imperfect helical lattice.
    • Filament structure and assembly are influenced by molecular structure, oligomer formation, and nucleation/elongation.
    • Specific regions near the amino and carboxyl termini of the rod domain are critical for native filament production.
    • Molecular packing imperfections appear to play a significant role in filament assembly and dynamics.

    Conclusions:

    • The hierarchical assembly of intermediate filaments is governed by specific molecular interactions and lattice arrangements.
    • Imperfections in molecular packing are integral to the dynamic assembly process of intermediate filaments.
    • Further research into these packing dynamics can refine our understanding of filament function and dysfunction.