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Updated: Aug 8, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Identification and quantitation of intact diastereoisomeric benzodiazepine glucuronides in biological samples by
1Institute of Forensic Medicine, University of Tübingen, Germany.
Abstract:
A rapid and simple high-performance liquid chromatographic method for the simultaneous detection of intact glucuronides of different benzodiazepines is described. Separation of the diastereomers of the following benzodiazepine glucuronides can be achieved on a reversed-phase column (octadecyl or select B): oxazepam, nordiazepam, temazepam, lorazepam and 3-hydroxyprazepam. If the sample contains both S-lorazepam and R-temazepam glucuronides, or both S-temazepam and nordiazepam glucuronides, further separation on a beta-cyclodextrin column is required. The detection limit ranges between 5 and 10 ng of glucuronides per ml of plasma or urine, respectively.
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