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Lambda kil-mediated lysis requires the phage context

G R Reisinger1, A Rietsch, W Lubitz

  • 1Institute of Microbiology and Genetics, University of Vienna, Austria.

Virology
|April 1, 1993
PubMed
Summary
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The lambda kil gene causes premature cell lysis when chloramphenicol is added after prophage induction. However, this lysis effect requires the phage context, not just kil gene expression.

Area of Science:

  • Bacteriophage biology
  • Molecular genetics

Background:

  • The lambda bacteriophage's kil gene is implicated in premature lysis upon chloramphenicol treatment post-induction.
  • Understanding the precise role and regulation of kil is crucial for phage-host interaction studies.

Purpose of the Study:

  • To precisely localize the lambda kil gene's reading frame.
  • To investigate the function of the kil gene, particularly its role in cell lysis.
  • To determine if kil-mediated lysis is dependent on the phage context.

Main Methods:

  • Gene mapping to identify the kil reading frame (orf47).
  • Plasmid-based expression of the kil gene.
  • Phenotypic analysis including growth arrest, colony-forming units, and filamentation.
  • Chloramphenicol treatment assays to assess lysis.

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Main Results:

  • The kil reading frame (orf47) was localized, overlapping the cIII and gam genes.
  • Expression of the plasmid-borne kil gene led to growth arrest, reduced colony-forming units, and filament formation.
  • Chloramphenicol-induced lysis was not observed when kil was expressed from a plasmid, indicating a requirement for the phage context.

Conclusions:

  • The lambda kil gene's function in cell lysis is dependent on the presence of the phage.
  • Kil gene expression alone, without the phage context, causes growth defects but not lysis.
  • Further research is needed to elucidate the specific phage factors involved in kil-mediated lysis.