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Dobrava virus as a new Hantavirus: evidenced by comparative sequence analysis
S Y Xiao1, G Diglisic, T Avsic-Zupanc
1Disease Assessment Division, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702-5011.
Journal of Medical Virology
|February 1, 1993
Summary
A new hantavirus, Dobrava virus, was identified in Slovenia. Genetic analysis shows it is distinct from other known hantaviruses, likely representing a new species.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Hemorrhagic fever with renal syndrome (HFRS) cases were reported in Slovenia.
- A novel virus, Dobrava virus, was isolated from yellow-neck mice (Apodemus flavicollis).
Purpose of the Study:
- To investigate the genetic relationship of the Dobrava virus to other known hantaviruses.
- To determine if Dobrava virus represents a new hantavirus species.
Main Methods:
- Serological analysis and restriction enzyme digestion of PCR-amplified gene segments.
- Nucleotide sequencing of the medium (M) genome segment of Dobrava virus.
- Phylogenetic analysis based on pairwise sequence similarity.
Main Results:
- Dobrava virus showed a 41.7% nucleotide sequence homology with other hantaviruses.
- Phylogenetic analysis indicated that Dobrava virus is genetically distinct.
- The findings suggest Dobrava virus is a new virus within the Hantavirus genus.
Conclusions:
- Dobrava virus is a distinct genetic entity within the Hantavirus genus.
- This discovery expands the known diversity of hantaviruses.
- Further research is warranted to understand the pathogenicity and epidemiology of Dobrava virus.