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Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening06:00

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening

This protocol describes the assembly of a pneumatic system for the delivery of pressurized air to a needle during the process of needle beveling. The protocol further describes the beveling process for creating sharp microinjection needles and how to gauge the relative opening size of the...
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Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels08:12

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels

This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.
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A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy03:56

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy

This "two-step method" significantly improved the success rate of percutaneous transhepatic choledochoscopy and achieved a better prognosis of intrahepatic and extrahepatic...
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Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)05:39

Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)

Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case...
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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal...
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy11:27

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research...
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