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Updated: Aug 19, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Published on: May 5, 2014
Inhibitory effect of pyridobenzazoles on virus replication in vitro
T Chiba1, S Shigeta, Y Numazaki
1Department of Materials Engineering, Akita National College of Technology, Japan.
Abstract:
Twelve species of pyridobenzazoles and pyrimidobenzimidazole were examined as inhibitors of the replication of respiratory syncytial virus (RSV) in HeLa cells. From the pyridobenzazoles studied, 2-benzamido-4-cyano-1-oxo-1H,5H-pyrido[1,2-alpha]benzimidazol emerged as a potent inhibitor of the RSV. Based on its inhibitory effect on the cytopathogenicity of the RSV in HeLa cells, the 50% effective dose was found to be 0.95 micrograms/ml. The cytotoxicity of this compound for HeLa cells was examined by monitoring the incorporation of radiolabeled uridine into cellular RNA. The selectivity index was ca. 30, the same as Virazole.
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