Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Osteoclast ATP receptor activation leads to a transient decrease in intracellular pH

H Yu1, J Ferrier

  • 1Medical Research Council Group in Periodontal Physiology, University of Toronto, Ontario, Canada.

Journal of Cell Science
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heterogeneous populations of ES cells in the generation of a floxed Presenilin-1 allele.

Genesis (New York, N.Y. : 2000)·2000
Same author

Methylation of the human telomerase gene CpG island.

Cancer research·2000
Same author

Proto-oncogenes and p53 protein expression in normal cervical stratified squamous epithelium and cervical intra-epithelial neoplasia.

European journal of cancer (Oxford, England : 1990)·2000
Same author

Gene gun application in the generation of effector T cells for adoptive immunotherapy.

Cancer immunology, immunotherapy : CII·2000
Same author

Cutting edge: IL-15 costimulates the generalized Shwartzman reaction and innate immune IFN-gamma production in vivo.

Journal of immunology (Baltimore, Md. : 1950)·2000
Same author

Computer-based radiation safety training for hospital radiation workers.

Health physics·2000
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
Same journal

The nucleus-vacuole junction at a glance.

Journal of cell science·2026
Same journal

Loss of INPP5E affects photoreceptor outer segment membrane biogenesis in iPSC-derived human retinal organoids.

Journal of cell science·2026
Same journal

Brinker regulates reciprocal outcomes of BMP signal between stem cells and differentiating cells.

Journal of cell science·2026
Same journal

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
See all related articles

Extracellular adenosine triphosphate (ATP) causes a decrease in osteoclast intracellular pH (pHi), mediated by purinergic receptors and influenced by proton pumps and chloride/bicarbonate exchange. This pH change is independent of calcium signaling.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Osteoclasts are crucial for bone resorption.
  • Intracellular pH (pHi) regulation is vital for osteoclast function.
  • Purinergic signaling plays a role in cellular processes.

Purpose of the Study:

  • To investigate the effect of extracellular adenosine triphosphate (ATP) on osteoclast intracellular pH (pHi).
  • To elucidate the mechanisms underlying ATP-induced pHi changes in osteoclasts.
  • To determine the role of purinergic receptors, calcium signaling, ion exchangers, and proton pumps in this process.

Main Methods:

  • Osteoclast culture and pHi measurement using seminaphthofluorescein (SNAFL)-calcein and laser scanning confocal microscopy.
  • Application of various purinergic agonists and antagonists (e.g., ATP, ADP, suramin).

Related Experiment Videos

  • Manipulation of intracellular calcium levels using BAPTA/AM.
  • Inhibition of ion transport mechanisms, including Na+/H+ exchange and Cl-/HCO3- exchange.
  • Use of specific proton pump inhibitors (e.g., N-ethylmaleimide, vanadate, bafilomycin).

    • Main Results:

    • Extracellular ATP induced a transient decrease in osteoclast pHi, which was partially inhibited by suramin, suggesting P2 purinergic receptor involvement.
    • The ATP-induced pHi decrease was independent of intracellular calcium ([Ca2+]i) increases.
    • Inhibition of Na+/H+ exchange did not affect the ATP-induced pHi change.
    • Specific proton pump inhibitors (N-ethylmaleimide, vanadate) partially inhibited the ATP effect, while others (bafilomycin, DCC) had no effect.
    • Inhibition of Cl-/HCO3- exchange led to a sustained pHi increase and reduced the ATP-induced pHi decrease, highlighting its role in pHi homeostasis.

    Conclusions:

    • Extracellular ATP significantly impacts osteoclast pHi through mechanisms involving P2 purinergic receptors.
    • The observed pHi decrease is primarily mediated by ion transport, including proton pumps and Cl-/HCO3- exchange, rather than calcium signaling.
    • These findings provide insights into the regulation of osteoclast function and bone remodeling.