Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mixed-function oxidase activity in sepsis

C V Godellas1, J F Williams, P J Fabri

  • 1Surgical Service James A. Haley Veterans Hospital, Tampa, Florida 33612, USA.

The Journal of Surgical Research
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Positron re-emission, reflection, and diffraction from W(100) surface at very low energies.

Journal of physics. Condensed matter : an Institute of Physics journal·2023
Same author

Coma.

The Southern medical record·2022
Same author

Smoking habits of UK military personnel on deployment: Exercise SAIF SAREEA 3.

BMJ military health·2020
Same author

HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line.

In vitro cellular & developmental biology. Animal·2015
Same author

Influence of polar groups in binary polymer blends on positronium formation.

Physical review. E, Statistical, nonlinear, and soft matter physics·2013
Same author

Microbial contamination of dental unit waterlines: short- and long-term effects of flushing.

General dentistry·2012
Same journal

Emergency Surgical Cricothyrotomy Videos Online: Reliability and Training Quality Evaluation.

The Journal of surgical research·2026
Same journal

Management of Concomitant Traumatic Abdominal Vascular and Gastrointestinal Injuries: A Descriptive Study.

The Journal of surgical research·2026
Same journal

Article Processing Charges in General Surgery Journals: Implications for Equitable Publishing Access in Low-Income and Middle-Income Countries.

The Journal of surgical research·2026
Same journal

Association of Distal Internal Carotid Artery Tortuosity With Carotid Artery Stenting Outcomes.

The Journal of surgical research·2026
Same journal

Appendectomy and Subsequent Type 2 Diabetes - A National Cohort study.

The Journal of surgical research·2026
Same journal

Perceptions and Barriers to Intraoperative Non-technical Skills Feedback in General Surgery: A Mixed-Methods Study.

The Journal of surgical research·2026
See all related articles

Sepsis impairs liver function by decreasing mixed-function oxidase (MFO) activity, which worsens over time. Hyperoxia offers partial protection against this sepsis-induced hepatic dysfunction.

Area of Science:

  • Hepatology
  • Sepsis Research
  • Biochemistry

Background:

  • Hepatic dysfunction is a critical factor in mortality associated with multiple organ system failure.
  • The impact of sepsis duration on liver dysfunction requires further investigation.

Purpose of the Study:

  • To investigate the effect of sepsis duration on liver mixed-function oxidase (MFO) activity and lipid peroxidation.
  • To evaluate the potential protective role of hyperoxia in sepsis-induced hepatic dysfunction.

Main Methods:

  • Fulminant cecal ligation and puncture (CLP) peritonitis model in rats.
  • Liver tissue analysis at 18, 21, 24, and 27 hours post-CLP.
  • Measurement of cytochrome P450 concentration and activity (ethoxyresorufin and ethoxycoumarin metabolism).

Related Experiment Videos

  • Assessment of lipid peroxidation via malondialdehyde content.

    • Main Results:

    • Septic rats exhibited significant decreases in cytochrome P450 levels and activity, with severity increasing with sepsis duration.
    • Hyperoxia partially mitigated the reduction in MFO content and activity.
    • A trend towards increased lipid peroxidation was observed but did not reach statistical significance.

    Conclusions:

    • Sepsis leads to a progressive decline in hepatic MFO content and activity.
    • The observed decreases in MFO are not directly linked to oxygen-derived free radical production.
    • Hyperoxia demonstrates a protective effect against sepsis-induced MFO impairment in this model.