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A study of immunoactivity of retinal S-antigen in retinoblastoma
1Department of Ophthalmology, 2nd Clinical College, Norman Bethune University of Medical Sciences, Changchun, China.
Purposes:
To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations.
Methods:
Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody,MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 10 cases of retinoblastomas. S-antigen was localized by the biotinavidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with 1:100 dilution. The whole procedure could be finished within a few hours.
Results:
The S-antigen immunoactivity was observed in different patterns: the "normal" photoreceptor elements incorporated in 3 cases of growing tumors; 3 of 4 Fleurettes and E-W rosettes; and scattered tumor cells in 50% of the cases.
Conclusions:
The result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree of tumor differentiation as another tumor marker in retinoblastoma.