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Related Experiment Videos

Flow cytometer and imaging device used in combination

F Kubota1, H Kusuzawa, T Kosaka

  • 1Technology and Research Division, TOA Medical Electronics Co., Kobe, Japan.

Cytometry
|October 1, 1995
PubMed
Summary

This study introduces a novel flow cytometer with a CCD camera for particle imaging. The system effectively detects aggregated platelets and urine casts, combining flow cytometry and morphology for precise cell identification.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Flow cytometry is a powerful technique for cell analysis.
  • Current methods may struggle with identifying specific particle types or rare cells.
  • Integrating imaging capabilities can enhance flow cytometry's diagnostic potential.

Purpose of the Study:

  • To develop and present a novel flow cytometer system equipped with a CCD camera for particle imaging.
  • To demonstrate the system's capability in detecting specific biological particles like aggregated platelets and urine casts.
  • To combine flow cytometric data with morphological information for improved cell identification and quantification.

Main Methods:

  • A custom-built flow cytometer utilizing an argon ion laser for analysis and a diode-pumped pulse laser for CCD imaging.

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  • Linking acquired particle images to their corresponding positions on the flow cytometry scattergram.
  • Utilizing a fast flow rate for efficient particle detection and analysis.

    • Main Results:

    • The system successfully generates and links images of selected particles to scattergram data.
    • Detection of aggregated platelets in blood samples and casts in urine samples was achieved.
    • Sufficient image resolution allowed for the recognition of aggregated platelets and casts.
    • Combined flow cytometric and morphological data enabled differentiation of cell types and identification of rare cells.

    Conclusions:

    • The developed flow cytometer with integrated CCD imaging offers enhanced particle analysis capabilities.
    • The system is effective for detecting clinically relevant particles such as aggregated platelets and urine casts.
    • The combined approach of flow cytometry and imaging provides a powerful tool for distinguishing cell populations and identifying rare events.