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Related Experiment Videos

Using GFP to see the light

D C Prasher1

  • 1USDA, Otis ANGB, MA 02542, USA.

Trends in Genetics : TIG
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

Green fluorescent protein (GFP) enables a unique reporter system due to its intense fluorescence. This species-independent protein does not require additional factors for its chromophore formation, making it easily detectable.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Green fluorescent protein (GFP) originates from the jellyfish Aequorea victoria.
  • GFP exhibits intense green bioluminescence.
  • The fluorescence is attributed to a chromophore formed from modified amino acids within the GFP polypeptide.

Purpose of the Study:

  • To highlight the utility of GFP as a reporter system.
  • To demonstrate the broad applicability of GFP in various experimental systems.

Main Methods:

  • Utilizing the gfp cDNA as a reporter gene.
  • Observing the intense fluorescence of the GFP gene product for detection.

Main Results:

  • GFP fluorescence formation is species-independent.

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  • GFP does not require additional factors for chromophore development.
  • The gfp cDNA serves as an easily detectable reporter system.

    • Conclusions:

    • GFP is a versatile and powerful reporter system in molecular biology.
    • The ease of detection and species independence of GFP facilitate its use across diverse organisms.
    • GFP applications are demonstrated in systems including Dictyostelium, plants, Drosophila, and mammalian cells.