Binding of dexamethasone by the subcellular fractions of mouse epidermis and dermis

The binding of the potent anticarcinogenic agent, dexamethasone, to mouse epidermal and dermal subcellular fractions was investigated. When applied to mouse skin, 3H-labeled dexamethasone remained associated, after extensive dialysis, with epidermal and dermal cytosol, microsomes, microsomes, mitochondria, and chromatin. The specific activity of dexamethasone binding to these fractions was from 2 to 5 times as high in the epidermis as in the dermis. Epidermal chromatin had the highest specific activity fo binding. Dexamethasone was not associated with the cytosol protein receptor which was previously found to specifically bind tumor promoters and polycyclic hydrocarbon carcinogens.