Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence and expression patterns of mouse SPR1: Correlation of expression with epithelial function

T Kartasova1, N Darwiche, Y Kohno

  • 1Department of Cancer Cell Research, University of Tokyo, Showa University School of Dentistry, Japan.

The Journal of Investigative Dermatology
|February 1, 1996
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An elastic pendulum thrust stand for evaluating six-degree-of-freedom thrust of 10 mN-class propulsion systems.

The Review of scientific instruments·2025
Same author

Direct measurement of 1-mN-class thrust and 100-s-class specific impulse for a CubeSat propulsion system.

The Review of scientific instruments·2020
Same author

Combined effects of light and water availability on photosynthesis and growth of Arisaema heterophyllum in the forest understory and an open site.

Oecologia·2017
Same author

Combined effects of light and water availability on photosynthesis and growth of Arisaema heterophyllum in the forest understory and an open site.

Oecologia·2017
Same author

Expressions of isopeptide bonds and corneodesmosin in middle ear cholesteatoma.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2016
Same author

Wearable blood flowmeter appcessory with low-power laser Doppler signal processing for daily-life healthcare monitoring.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2015

Small proline-rich (SPR) proteins like SPR1a are crucial for building cell envelopes in stratified squamous epithelia. Their expression in specific tissues suggests a role in structural flexibility and thickness.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Dermatology

Background:

  • Stratified squamous epithelia differentiation involves cornified cell envelope formation.
  • Small proline-rich (SPR) proteins act as cross-linking agents in these envelopes.
  • Understanding SPR protein function is key to epithelial biology.

Purpose of the Study:

  • To investigate the evolution and expression of mouse SPR1 proteins.
  • To characterize the structural conservation and tissue distribution of SPR1a and SPR1b.
  • To elucidate the role of SPR1a in epithelial cell envelope construction.

Main Methods:

  • cDNA isolation and sequencing of mouse SPR1a and SPR1b.
  • Northern blot analysis for gene expression.
  • In situ hybridization and immunohistochemistry for protein localization.

Related Experiment Videos

Main Results:

  • Mouse SPR1a and SPR1b share conserved structural features with human orthologs.
  • SPR1a expression is detected in fetal periderm, anagen hair follicles, and thickened epidermis.
  • High SPR1a levels are found in forestomach, penis, papillomas, tongue, esophagus, and vagina epithelia.

Conclusions:

  • SPR1a is widely involved in cell envelope formation in cornifying epithelia.
  • SPR1a contributes to epithelial thickness and flexibility.
  • SPR1 protein content influences the mechanical properties of cell envelopes.