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Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide
N Noguchi1, A Emura, H Matsuyama
1Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Antimicrobial Agents and Chemotherapy
|October 1, 1995
Abstract:
The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.