Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Random-breakage mapping method applied to human DNA sequences

M Löbrich1, B Rydberg, P K Cooper

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA.

Nucleic Acids Research
|May 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tribotronic control of friction in oil-based lubricants with ionic liquid additives.

Physical chemistry chemical physics : PCCP·2016
Same author

The rate of strand separation in alkali of DNA of irradiated mammalian cells. 1975.

Radiation research·2012
Same author

Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography.

European radiology·2010
Same author

[X-ray-induced DNA double-strand breaks after angiographic examinations of different anatomic regions].

RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2009
Same author

DNA double-strand breaks: their cellular and clinical impact?

Oncogene·2007
Same author

DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage.

Advances in space research : the official journal of the Committee on Space Research (COSPAR)·2005
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

The random-breakage mapping method accurately maps DNA fragment locations. This technique uses radiation-induced breaks to precisely position genetic probes on human chromosomes, confirming its validity.

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomic Mapping

Background:

  • Accurate gene mapping is crucial for understanding genetic diseases.
  • Existing methods may have limitations in precisely locating DNA sequences within large fragments.

Purpose of the Study:

  • To validate and apply the random-breakage mapping method for precise DNA sequence localization.
  • To map specific probes on human chromosomes X and 21.

Main Methods:

  • Utilized the random-breakage mapping technique involving NotI digestion, pulsed-field gel electrophoresis, and Southern blotting.
  • Employed DNA probes for hybridization to identify specific single-copy sequences.
  • Analyzed Southern blot smear patterns for discontinuities indicating fragment ends.
Keywords:
NASA Discipline Radiation HealthNon-NASA Center

Related Experiment Videos

Main Results:

  • Confirmed the mapped position of probe DXS1327 on the X chromosome.
  • Successfully positioned probes D21S1 and D21S15 on chromosome 21.
  • Resolved ambiguity regarding the location of probe D21S11 on chromosome 21, confirming its proximity to D21S1.

Conclusions:

  • The random-breakage mapping method is a valid and effective technique for high-resolution DNA mapping.
  • This method accurately determines the position of genetic markers relative to restriction fragment ends.
  • The study successfully mapped multiple probes, contributing to a more refined human genome map.