[Effect of pyruvate and alpha-ketoglutarate on protein and lipid synthesis in rat tissues]

The in vivo and in vitro experiments on rats showed that administration of pyruvate and alpha-ketoglutarate causes stimulation of protein and lipid synthesis displayed in intensification of incorporation of the label from 1-14C lysine and NaCH14O3 into the mentioned components of the liver, kidneys, spleen, myocardium and femur muscles tissues. The stimulating effect of the studied ketoacids is much higher when incubating the slices in the Krebs-Ringer medium on the bicarbonate buffer than on the phosphate one (pH 7.4), that evidences for an important role of carbonic acid in biosynthetic processes.