[Amperometric titration of erythrocyte membrane sulfhydryl groups]

The best conditions are selected for amperometric titration of erythrocyte membranes SH-groups by means of silver ions. On the basis of the experimental data, two types of SH-groups according to their capacity of binding Ag+ are found in the membranes. Dynamics of transition of SH-groups of types I into SH-groups of type II is studied. The chemical model explaining the presence of two bends in the course of the membranes amperometric titration curve is created. Calculations were made for the products of R-S-Ag solubility for SH-groups of type I and SH-groups of types I and II as well as for entropy and heat of formation of silver compounds with the membranes sulphydryl groups.