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Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
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[Fluorometric determination of amide protein groups]

I V Shepotinovskaia, I N Nazarova, N Ia Umanskaia

    Klinicheskaia Laboratornaia Diagnostika
    |May 1, 1995
    PubMed

    Abstract:

    A new fluorometric method for detection of amide protein groups is proposed. The method has a number of advantages over the routine phenol hypochlorite method, this permitting its wider application in clinical and laboratory practice. The suggested method may be used for direct detection of ammonium in biological samples.

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    In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is broad and...

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