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Updated: Aug 2, 2026
12:28
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Published on: May 3, 2015
Structure and function of kinetochores in budding yeast
1European Molecular Biology Laboratory, Heidelberg, Germany.
Annual Review of Cell and Developmental Biology
|January 1, 1995
Summary
This review details budding yeast centromeres and kinetochores, focusing on their molecular analysis and the proteins involved in chromosome movement during cell division. It explores how these structures attach chromosomes to the mitotic spindle.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Kinetochores are essential DNA-protein structures mediating chromosome attachment and movement via the mitotic spindle.
- Budding yeast (Saccharomyces cerevisiae) offers a simplified model for detailed molecular analysis of centromeres and kinetochores.
Purpose of the Study:
- To review the molecular organization and function of centromeres and kinetochores in Saccharomyces cerevisiae.
- To describe methods for analyzing centromeric DNA and identifying centromere-binding proteins.
Main Methods:
- Analysis of chromosome movement and microtubule attachment in living yeast cells.
- Cloning and molecular analysis of centromeric DNA.
- Biochemical and genetic isolation of centromere-binding proteins.
Main Results:
- Detailed molecular analysis of budding yeast centromeres is feasible due to their simplicity.
- Identification of key centromere-binding proteins through integrated biochemical and genetic approaches.
- In vitro studies provide insights into microtubule attachment mechanisms relevant to in vivo events.
Conclusions:
- A speculative model for budding yeast centromere organization is proposed.
- Future research directions for understanding centromere and kinetochore function are considered.
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