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[Effect of hyaluronic acid-stimulating factor on scar formation in wound healing process of deep partial thickness
1Research Institute of Trauma and Burn Surgery, Changhai Hospital, Second Military Medical University, Shanghai.
Abstract:
Hyaluronic acid-stimulating factor (HASF) was purified from the amniotic fluid and fetal serum of the gestational rabbit by means of affinity chromatography. After topical application of HASF on the deep partial thickness burn wounds, the level of hyaluronic acid (HA) in wound exudate was determined daily during the early period of healing. One month after wound healing, the local skin was harvested for collagen analysis. Histologic study was made to check the outline of collagen fibrils. The HASF was also used in rabbit fibroblast culture. The HA level contained in the supernatant was assayed. Result showed that HASF could elevate and maintain the HA level in the extracellular matrix of burn wound. In vitro, HASF was able to stimulate the fibroblast to produce hualuronic acid. The presence of HA in extracellular matrix then modulated collagen synthesis in the process of wound repair resulting in a higher content of type III collagen, thus bringing about a scarless healing of the deep patrial thickness burns.