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Myoblast seeding in a collagen matrix evaluated in vitro
P B van Wachem1, M J van Luyn, M L da Costa
1Laboratory for Cell Biology and Electron Microscopy, University of Groningen, The Netherlands.
Journal of Biomedical Materials Research
|March 1, 1996
Summary
Seeding muscle cells into dermal sheep collagen (DSC) biomaterial is feasible. The cells survived, differentiated into muscle fibers, and formed new extracellular matrix, suggesting potential for muscle tissue regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Collagens are promising biomaterials for soft tissue reconstruction.
- Dermal sheep collagen (DSC) supports fibroblast infiltration but not muscle regeneration in abdominal wall models.
- Muscle regeneration via DSC may be achievable by seeding muscle cells.
Purpose of the Study:
- To investigate the feasibility of seeding mouse C2C12 myoblasts into DSC disks.
- To assess cell distribution, survival, differentiation, and extracellular matrix formation within DSC.
Main Methods:
- Mouse C2C12 myoblasts were seeded into DSC disks using a syringe.
- Cells were cultured in growth medium then switched to differentiation medium.
- Cell morphology, distribution, adhesion, myotube formation, and extracellular matrix deposition were analyzed over 7 days.
Main Results:
- Approximately 85% of seeded cells were well distributed within DSC disks.
- Cell survival rate was 45% after 7 days.
- Seeded myoblasts differentiated into myotubes and deposited collagen and elastin, forming extracellular matrix.
Conclusions:
- Myoblast seeding into DSC is a viable method for achieving cell distribution and survival.
- The surviving cells can differentiate into functional myotubes and create new extracellular matrix.
- DSC holds potential as a scaffold for muscle tissue regeneration.